insulin like growth factor 2 igf2 levels Search Results


myc igf2bp2  (Shanghai Korain Biotech Co Ltd)
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growth factor 2 elisa kit  (Elabscience Biotechnology)
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primary antibodies against imp1  (Boster Bio)
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Boster Bio primary antibodies against imp1
Figure 2. <t>IMP1</t> regulates the localization of SNHG15 at the cell protrusions. (A) FISH was per- formed to detect the subcellular localization of SNHG15 in WT BT-549 cells (upper panel) and SNHG15-expressing BT-549 cells (lower panel). The arrowheads indicate localized SNHG15 at the cell protrusions. Scale bar: 10 µm. (B) FISH indicated that, in comparison with WT MDA-MB-231 cells (upper panel), IMP1 expression greatly increased the localization of SHNG15 at cell protrusions (lower panel). Scale bar: 10 µm. (C) A bar graph indicates the percentage of protrusion-localized SNHG15 in tested cells. Localization was increased to 70% from 40% when ectopic IMP1 was ex- pressed. About 80–100 cells were counted in each group. ** p < 0.01. WT: cell origin. IMP1: cells expressing ectopic IMP1. Control: cells transfected with an empty plasmid. (D) RT-qPCR showed that IMP1 expression does not affect cellular levels of SNHG15.
Primary Antibodies Against Imp1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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insulin like growth factor  (Cusabio)
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Cusabio insulin like growth factor
Figure 2. <t>IMP1</t> regulates the localization of SNHG15 at the cell protrusions. (A) FISH was per- formed to detect the subcellular localization of SNHG15 in WT BT-549 cells (upper panel) and SNHG15-expressing BT-549 cells (lower panel). The arrowheads indicate localized SNHG15 at the cell protrusions. Scale bar: 10 µm. (B) FISH indicated that, in comparison with WT MDA-MB-231 cells (upper panel), IMP1 expression greatly increased the localization of SHNG15 at cell protrusions (lower panel). Scale bar: 10 µm. (C) A bar graph indicates the percentage of protrusion-localized SNHG15 in tested cells. Localization was increased to 70% from 40% when ectopic IMP1 was ex- pressed. About 80–100 cells were counted in each group. ** p < 0.01. WT: cell origin. IMP1: cells expressing ectopic IMP1. Control: cells transfected with an empty plasmid. (D) RT-qPCR showed that IMP1 expression does not affect cellular levels of SNHG15.
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igf 2r  (Boster Bio)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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insulin-like growth factor-2 (igf-2)  (Paul N Gardner)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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forward and reverse primers for insulin growth factor 2 (igf2)  (PrimerDesign Inc)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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elisa kit  (Elabscience Biotechnology)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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ek1326 human igf2r picokine tm elisa kit 1 kit 96t for igf2r  (Boster Bio)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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rat insulin  (Cusabio)
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The primers and TaqMan probes of <t> IGF, </t> ER genes used for RT-PCR
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Image Search Results


Figure 2. IMP1 regulates the localization of SNHG15 at the cell protrusions. (A) FISH was per- formed to detect the subcellular localization of SNHG15 in WT BT-549 cells (upper panel) and SNHG15-expressing BT-549 cells (lower panel). The arrowheads indicate localized SNHG15 at the cell protrusions. Scale bar: 10 µm. (B) FISH indicated that, in comparison with WT MDA-MB-231 cells (upper panel), IMP1 expression greatly increased the localization of SHNG15 at cell protrusions (lower panel). Scale bar: 10 µm. (C) A bar graph indicates the percentage of protrusion-localized SNHG15 in tested cells. Localization was increased to 70% from 40% when ectopic IMP1 was ex- pressed. About 80–100 cells were counted in each group. ** p < 0.01. WT: cell origin. IMP1: cells expressing ectopic IMP1. Control: cells transfected with an empty plasmid. (D) RT-qPCR showed that IMP1 expression does not affect cellular levels of SNHG15.

Journal: International journal of molecular sciences

Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.

doi: 10.3390/ijms242115600

Figure Lengend Snippet: Figure 2. IMP1 regulates the localization of SNHG15 at the cell protrusions. (A) FISH was per- formed to detect the subcellular localization of SNHG15 in WT BT-549 cells (upper panel) and SNHG15-expressing BT-549 cells (lower panel). The arrowheads indicate localized SNHG15 at the cell protrusions. Scale bar: 10 µm. (B) FISH indicated that, in comparison with WT MDA-MB-231 cells (upper panel), IMP1 expression greatly increased the localization of SHNG15 at cell protrusions (lower panel). Scale bar: 10 µm. (C) A bar graph indicates the percentage of protrusion-localized SNHG15 in tested cells. Localization was increased to 70% from 40% when ectopic IMP1 was ex- pressed. About 80–100 cells were counted in each group. ** p < 0.01. WT: cell origin. IMP1: cells expressing ectopic IMP1. Control: cells transfected with an empty plasmid. (D) RT-qPCR showed that IMP1 expression does not affect cellular levels of SNHG15.

Article Snippet: Primary antibodies against IMP1, GAPDH and nucleolin (NCL) were purchased from Cell Signaling Co. (Danvers, MA, USA); CDH2 and CDH1 antibodies were purchased from Boster Biological Technology Co. (Wuhan, China).

Techniques: Expressing, Comparison, Control, Transfection, Plasmid Preparation, Quantitative RT-PCR

Figure 3. Localization of SNHG15 at the cell protrusions increases cell invasive potential. (A) The full-length SNHG15 and three dissected fragments of SNHG15 are separately tagged with six MS2 repeats. The relative position of the truncated SNHG15 is shown. The black box indicates the “ACACCC” motif for IMP1 binding. (B) FISH experiments showed that deletion of the “ACACCC” motif (Mut SNHG15) prevented SNHG15 from localizing at the cell protrusions. The arrowhead indicates cell-protrusion-localized SNHG15. Scale bar: 10 µm. (C) A bar graph shows that the cell population with protrusion-localized SNHG15 was largely decreased when the “ACACCC” motif was absent. ** p < 0.01. (D) Localization of the full-length and three truncated fragments of SNHG15 was determined by FISH. Results indicated that T1 and T3, but not T2, were still able to localize at the cell protrusions. About 80–100 cells were counted in each group. ** p < 0.01. (E) Transwell assays showed that, compared to the cells expressing full-length SNHG15, all three stable cell lines expressing truncated SNHG15 displayed lower invasive potentials. ** p < 0.01.

Journal: International journal of molecular sciences

Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.

doi: 10.3390/ijms242115600

Figure Lengend Snippet: Figure 3. Localization of SNHG15 at the cell protrusions increases cell invasive potential. (A) The full-length SNHG15 and three dissected fragments of SNHG15 are separately tagged with six MS2 repeats. The relative position of the truncated SNHG15 is shown. The black box indicates the “ACACCC” motif for IMP1 binding. (B) FISH experiments showed that deletion of the “ACACCC” motif (Mut SNHG15) prevented SNHG15 from localizing at the cell protrusions. The arrowhead indicates cell-protrusion-localized SNHG15. Scale bar: 10 µm. (C) A bar graph shows that the cell population with protrusion-localized SNHG15 was largely decreased when the “ACACCC” motif was absent. ** p < 0.01. (D) Localization of the full-length and three truncated fragments of SNHG15 was determined by FISH. Results indicated that T1 and T3, but not T2, were still able to localize at the cell protrusions. About 80–100 cells were counted in each group. ** p < 0.01. (E) Transwell assays showed that, compared to the cells expressing full-length SNHG15, all three stable cell lines expressing truncated SNHG15 displayed lower invasive potentials. ** p < 0.01.

Article Snippet: Primary antibodies against IMP1, GAPDH and nucleolin (NCL) were purchased from Cell Signaling Co. (Danvers, MA, USA); CDH2 and CDH1 antibodies were purchased from Boster Biological Technology Co. (Wuhan, China).

Techniques: Binding Assay, Expressing, Stable Transfection

The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques:

The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Control

The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Immunostaining, Control, Positive Control

The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing, Control